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Oceanography

New PDF release: Algal Culturing Techniques

By Robert A. Andersen

ISBN-10: 0120884267

ISBN-13: 9780120884261

Algal Culturing Techniques is a complete reference on all points of the isolation and cultivation of marine and freshwater algae, together with seaweeds. it truly is divided into seven components that conceal heritage, media instruction, isolation and purification innovations, mass culturing suggestions, mobile counting and progress dimension ideas, and stories on issues and purposes of algal tradition concepts for environmental investigations.

Algal Culturing Techniques used to be built to function either a brand new textbook and key reference for phycologists and others learning aquatic platforms, aquaculture and environmental sciences. scholars of algal ecology, marine botany, marine phycology, and microbial ecology will benefit from the hands-on technique for culturing a number of algae from clean and marine waters. Researchers in undefined, resembling aquaculture, pharmaceutical, foodstuffs, and biotechnology businesses will locate an authoritative and complete reference.

  • Sponsored through the Phycological Society of America
  • Features colour photos and illustrations throughout
  • Describes culturing tools starting from the attempt tube to outdoors ponds and coastal seaweed farms
  • Details isolation recommendations starting from conventional micropipette to automatic move cytometeric methods
  • Includes purification, progress, upkeep, and cryopreservation techniques
  • Highlights tools for estimating algal populations, progress premiums, keeping apart and measuring algal pigments, and detecting and culturing algal viruses
  • Features a accomplished appendix of approximately 50 algal tradition medium recipes
  • Includes a word list of phycological terms

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Extra info for Algal Culturing Techniques

Sample text

Glassware should be autoclaved, clean glassware and plasticware should be stored in closed cupboards, and open vessels should be covered. One should also be aware of potential toxicity from tubing and other materials. Bernhard was one of the first to call attention to the inhibitory effects of some culture materials by testing more than 50 types on phytoplankton and zooplankton (Bernhard and Zattera 1970, Bernhard 1977). A later study examined latex tubings of rubber, polyvinyl chloride (Tygon), and silicone, and Price et al.

In principle, the simplest comparison to make is whether the algae grow. Consult the culture collection Web sites for the most recent information on recipes and also to find the most appropriate medium for a particular strain. In practice, this is considerably complicated by the culture methods. For example, some investigators may maintain their culture collections quite conscientiously, transferring cultures weekly or more often. , a high exponential growth rate, m) could be favored. Under these conditions, a medium that was relatively poorly buffered or contained relatively low levels of macronutrients might be regarded as superior.

23:633–8. , and Pytkowicz, R. 1967. Preparation of artificial seawater. Limnol. Oceanogr. 12:176–9. King, J. , and Spotte, S. H. 1974. Marine Aquariums in the Research Laboratory. , Eastlake, Ohio. Kinne, O. 1976. Cultivation of marine organisms. , ed. Marine Ecology, Vol III. Cultivation. John Wiley & Sons, Toronto, pp. 29–37. Levring, T. 1946. Some culture experiments with Ulva lactuca. Kgl. Fysiogr. Sallsk. Hdl. 16:45–56. Lewin, J. 1966. Silicon metabolism in diatoms. V. Germanium dioxide, a specific inhibitor of diatom growth.

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Algal Culturing Techniques by Robert A. Andersen


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